Figure 3.

The difference of miR-27a expression between normal gastric mucosal cells and GC cells and the expression of miR-27a in GC cells after interfering with the expression of miR-27a

(A) qRT-PCR was used to detect the expression of miR-27a in GC cells and normal gastric mucosal cells; (B) detection of transfection efficiency of miR-27a mimics in BGC-823 and MKN45 cells by qRT-PCR; (C) detection of transfection efficiency of miR-27a inhibitors in SGC-7901 and AGS by qRT-PCR; the experiment was repeated in triplicates; the comparison among multiple groups was analyzed by one-way ANOVA; the Fisher’s LSD-t was used for pairwise comparison; *, P < 0.05 compared with normal gastric mucosa cell or the blank group.