Figure 6.

The difference of SFRP1 expression between normal gastric mucosa cells and gastric carcinoma cells and the analysis of the expression of SFRP1 and the targeting relationship between miR-27a and SFRP1 in GC cells after interfering with the expression of miR-27a

(A) Detection of mRNA expression of SFRP1 in various cells by qRT-PCR; (B) detection of SFRP1 protein expression in various cells by Western blot analysis; (C) effect of miR-27a mimics on SFRP1 mRNA expression by qRT-PCR; (D) effect of miR-27a mimics on SFRP1 protein expression detected by Western blot analysis; (E) effect of miR-27a inhibitors on SFRP1 mRNA expression by qRT-PCR; (F) effect of miR-27a inhibitors on SFRP1 protein expression detected by western blot analysis; (G) online prediction software and luciferase activity determination to verify the targeting relationship between miR-27a and SFRP1; the experiment was repeated in triplicates; the comparison among two or multiple groups was analyzed by the t test or the one-way ANOVA; the Fisher’s LSD-t was used for pairwise comparison; *, P < 0.05 compared with GES-1 cells or the mimics NC group.