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. 2019 May 21;39(5):BSR20181175. doi: 10.1042/BSR20181175

Table 1.

Primer sequence

Gene Sequence
miR-27a F: 5′-TTCACAGTGGCTAAG-3′
R: 5′-GTGCAGGGTCCGAGGT-3′
U6 F: 5′- CTCGCTTCGGCAGCACA-3′
R: 5′-AACGCTTCACGAATTTGCGT-3′
SFRP1 F: 5′-CGAGTTTGCACTGAGGATGA-3′
R: 5′-CAGCACAAGCTTCTTCAGGTC-3′
GAPDH F:5′-TGGGTGTGAACCATGAGAAG-3′
R: 5′-GTGTCGCTGTTGAAGTCAGA-3′

Note: F, forward; GAPDH, glyceraldehyde phosphate dehydrogenase; miR-27a, microRNA-27a; R, reverse; SFRP1, secreted frizzled related protein 1.