Table 1.
Gene | Sequence |
---|---|
miR-27a | F: 5′-TTCACAGTGGCTAAG-3′ |
R: 5′-GTGCAGGGTCCGAGGT-3′ | |
U6 | F: 5′- CTCGCTTCGGCAGCACA-3′ |
R: 5′-AACGCTTCACGAATTTGCGT-3′ | |
SFRP1 | F: 5′-CGAGTTTGCACTGAGGATGA-3′ |
R: 5′-CAGCACAAGCTTCTTCAGGTC-3′ | |
GAPDH | F:5′-TGGGTGTGAACCATGAGAAG-3′ |
R: 5′-GTGTCGCTGTTGAAGTCAGA-3′ |
Note: F, forward; GAPDH, glyceraldehyde phosphate dehydrogenase; miR-27a, microRNA-27a; R, reverse; SFRP1, secreted frizzled related protein 1.