Table 3.
Approximate composition of DUV resonant macromolecular components in an average cell of E. coli during exponential growth with a doubling time of 40 min, based on a cellular volume of 0.9 μm3 (Neidhardt et al., 1990).
| Aromatic | ||||
|---|---|---|---|---|
| % cell | Weight per | units per | ||
| Macromolecule | mass | cell (fg) | % mol | cell (x106) |
| Protein 1 | 16.5 | 165 | 65.2 | |
| Phe | 3.46 | 31.8 | ||
| Trp | 1.06 | 9.74 | ||
| Tyr | 2.58 | 23.6 | ||
| RNA 2 | 6 | 60 | 106 | |
| A | 24.8 | 26.2 | ||
| C | 21.8 | 23.0 | ||
| G | 32.4 | 34.2 | ||
| U | 21.0 | 22.2 | ||
| DNA 3 | 0.9 | 9 | 16.6 | |
| A | 24.5 | 4.07 | ||
| C | 24.6 | 4.08 | ||
| G | 26.2 | 4.35 | ||
| T | 24.7 | 4.10 | ||
| Metabolites 4 | 7 | 70 | Concentration (mM) | 36.4 |
| A | 16.0 | 10.7 | ||
| C | 2.75 | 1.84 | ||
| G | 5.85 | 3.91 | ||
| T | 4.98 | 3.33 | ||
| U | 22.7 | 15.2 | ||
| Phe | 2.00 | 0.012 | ||
| Trp | 1.33 | 0.0008 | ||
| Tyr | 3.22 | 0.019 | ||
| Other | 5.7 | 57 | ||
| Water | 63.9 | 639 | ||
| Total | 100 | 1000 | 224 |
(1) Calculated based on 3 × 106 proteins per cell (Neidhardt et al., 1990), cumulative weight derived from Neidhardt et al. (1990), amino acid abundance from the proteome-pl database (Kozlowski, 2017), and the molecular mass of each amino acid. (2) Calculated using the cumulative weight derived from Neidhardt et al. (1990), the RNA composition reported by Nierlich (1972) and the individual masses of the ribonucleotide monophosphates. (3) Calculated using the cumulative weight derived from Neidhardt et al. (1990), the DNA composition from Blattner et al. (1997) and the individual masses of the deoxyribonucleotide monophosphates. (4) Calculated from concentrations of 103 free metabolites given by Bennett et al. (2009), grouped according to constituent aromatic unit. Bold values indicate totals for each macromolecule class.