Figure 3.

Nicotinic acetylcholine receptor (nAChR) responses to isoarecolone. Oocytes expressing human α7, α3β4, α4β2 nAChR subunits, mouse muscle α1β1εδ subunits, or the human α4β2α4β2α3 concatamer³³ were first evaluated for their responses to two control applications to ACh, and then for their responses to the analogs applied at 100 µM. Acetylcholine (ACh) controls were 60 µM for the α7-expressing cells, 100 µM for α3β4, and 30 µM for the other subtypes. Responses were normalized relative to the average of the two initial ACh applications from the same cells. Responses of the α7 receptors were calculated as net charge³⁴ and as peak current for the other subtypes. Additionally, the α7 receptors were also tested with isoarecolone co-applied with 10 µM of the PAM PNU-120596, and those data are indicated by the asterisk. (Note that these are scaled on the same left-hand y-axis.)