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. Author manuscript; available in PMC: 2020 May 17.
Published in final edited form as: ACS Chem Biol. 2019 Apr 17;14(5):959–965. doi: 10.1021/acschembio.9b00150

Figure 2. Engineering of a LumiLuc luciferase.

Figure 2.

(a) Procedure to derive LumiLuc from teLuc. (b) Illustration of the putative substrate-binding site and LumiLuc mutations from teLuc. CTZ is shown as spheres and mutated residues are presented in sticks. (c) Bioluminescence of pyCTZ, 6pyDTZ, or 8pyDTZ in the presence of either teLuc or LumiLuc. (d) Normalized bioluminescence emission spectra of pyCTZ, 6pyDTZ, and 8pyDTZ in the presence of LumiLuc.