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. 2019 May 20;10:135. doi: 10.1186/s13287-019-1250-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 1 — a Typical morphology of spermatogonia: rounded or oval cells and spiral nucleus related to the cytoplasm (circle). b, c Microscopic appearance of the formation of germ cell clumps (arrow) in small groups of cells (dotted circle) and continuous proliferation of clump-forming cells (asterisk) (2 days and 15 days after in vitro culture of cSSCs). d, e Formation of clumps by cells in suspension (arrow) among Sertoli cells (asterisk). (Scale bar = 100 μm)