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. 2019 May 20;10:135. doi: 10.1186/s13287-019-1250-9

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© The Author(s). 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 3 — Flow cytometric and fluorescence analysis of cSSCs. a–ai GFP+ cSSCs appear as isolated cells or form germ cell clumps (circle). aii Fluorescence analysis of cSSCs showing GFP positivity after transduction (circle). B Flow cytometry was used to evaluate the efficiency rate of the transduction of cSSCs with GFP compared with the control group. The control group (histogram) was GFP negative. The histogram shows that in the treated group, only 10.4% of the cSSC population was GFP+. (Scale bar = 100 μm)