Table 1.

2DL DEG between R and S NIL that were further characterized by RT-qPCR

2DL DEG Name (IWGSC release 2.25)	Annotation	IWGSC RefSeq v1.0e			Expression pattern	RT-qPCR characterization
		Name	Start	End		In 3 pairs of NIL	In Wuhan 1, Nyubai	In DH population
Traes_2DL_03CAA3B80a	copper-transporting ATPase PAA2, chloroplastic	TraesCS2D01G495500LC	493,192,302	493,195,914	2	yes	yes	yes
Traes_2DL_0A96AAC5Ba	Sodium transporter protein	TraesCS2D02G428300	540,161,984	540,166,078	24	yes
Traes_2DL_0AB0ABFD5	Malate synthase	TraesCS2D02G344200	440,325,740	440,328,360	27	yes
Traes_2DL_179570792a	WD-40 repeat family protein	TraesCS2D02G440500	550,630,373	550,636,721	1	yes	yes	yes
Traes_2DL_3BBD24259a	Smr domain-containing protein	TraesCS2D02G415100	529,007,465	529,012,316	12	yes
Traes_2DL_3F691BC13	Receptor kinase	TraesCS2D02G599300	650,752,287	650,757,581	9	yes
Traes_2DL_44552FCF2	DnaJ / Sec63 Brl domains-containing protein	TraesCS2D02G275600	345,248,090	345,248,281	23	yes
Traes_2DL_7B0056729	Serine incorporator	TraesCS2D02G347800	445,890,982	445,897,188	20	yes
Traes_2DL_848E9C4A6a	Heat shock transcription factor	TraesCS2D02G399000	512,041,712	512,043,335	36	yes
Traes_2DL_89A313AC3	Glycin-rich RNA-binding protein	TraesCS2D02G302400	385,568,830	385,570,145	28	yes	yes
Traes_2DL_BB6F31152	Receptor protein kinase	TraesCS2D02G248200	291,952,536	291,956,775	9	yes
Traes_2DL_E301DD433a	BTB/POZ and TAZ domain-containing protein 2	TraesCS2D02G339900	434,138,340	434,140,330	31	yes
Traes_2DL_F784FBAA3a	2-oxoglutarate (2OG) and Fe(II)-dependent oxygenase	TraesCS2D02G392900	500,791,603	500,793,692	24	yes
Traes_2DL_07F08C844a,b	cysteine-rich/transmembrane domain A-like protein	TraesCS2D02G417400	531,711,723	531,712,483	23		yes	yes
Traes_2DL_37D967CFCa,b	CC-NBS-LRR protein	TraesCS2D02G380500	484,858,484	484,862,886	23		yes	yes
Traes_2DL_382370E3B	Pectinesterase	TraesCS2D02G322500	413,777,752	413,779,866	18		yes	yes
Traes_2DL_5642F7EAC	Receptor-like protein kinase	TraesCS2D02G263100	320,280,150	320,283,723	10		yes
Traes_2DL_647B61E84	1,4-alpha-glucan-branching enzyme	TraesCS2D02G290800	372,924,177	372,935,106	7		yes
Traes_2DL_892F83E0B	Sec14p-like phosphatidylinositol transfer protein	TraesCS2D02G335700	429,053,162	429,057,812	18		yes
Traes_2DL_8A90A6C4Fa	Ankyrin repeat protein	TraesCS2D02G395500	506,771,094	506,784,989	39		yes
Traes_2DL_8F75414FD	Protein kinase	TraesCS2D02G301200	383,845,349	383,849,177	9		yes
Traes_2DL_A208876FE	Elongation factor 1-alpha	TraesCS2D02G320200	411,416,487	411,417,559	5		yes	yes
Traes_2DL_B7ABC1CB9a,b	Hypoxia-responsive protein	TraesCS2D02G438000	547,762,760	547,764,965	23		yes	yes
Traes_2DL_BA1B746DF	MLP protein	TraesCS2D02G328200	421,480,422	421,481,366	9		yes
Traes_2DL_EDAEA1357a,b	Acidic endochitinase	TraesCS2D02G450600	560,399,016	560,400,258	31		yesg
Traes_2DL_FA7A15F63	Glycerophosphodiester phosphodiesterase	TraesCS2D02G332500	425,740,309	425,746,371	10		yes
UN25696c,d	Uncharacterized protein	(No gene model)f	446,264,922	446,266,534		yes	yes	yes
TaWRKYc Traes_2DL_B8483F711	WRKY transcription factor	TraesCS2D02G489700	588,676,152	588,677,693			yes	yes
TaACTc,d	Agmatine coumaroyltransferase-2	TraesCS2D02G490400	589,282,163	589,283,855			yes	yes
NPR1-likec Traes_2DL_14C8A084C	Receptor-like protein kinase	TraesCS2D02G572000	638,143,208	638,148,768			yes	yes

^aDEG physically located in 5 LOD interval for FHB resistance 2DL QTL, based on IWGSC RefSeq v1.0 [38, 47]

^bDEG with log₂ FC > 1.5/<−1.5 for Wuhan 1/Nyubai at 2dai with F. graminearum

^cGenes proposed to contribute to the 2DL QTL for FHB resistance in literature [31, 33, 48]

^dNot annotated in Wheat Genome IWGSC release 2.25

^eBest BLAST hit in IWGSC RefSeq v1.0 [38, 47]

^f Not annotated in Wheat Genome IWGSC RefSeq v1.0 [38, 47]

^gNo expression was detected by RT-qPCR