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. 2019 Apr 25;14(5):1020–1029. doi: 10.1021/acschembio.9b00222

Figure 4.

Figure 4

Effects of ONC201 and TR-57 are prevented by ClpP knockdown in SUM159 cells. (a) Immunoblot of lysates from SUM159 cells transfected with a nontargeting siRNA (siCTRL) or siRNA targeting ClpP (siClpP) and treated with either 10 μM ONC201, 150 nM TR-57, or DMSO for 24 h. Blots shown are representative of three biological replicates. (b) Total protein synthesis measured in SUM159 cells transfected with siCTRL or siClpP after treatment with ONC201, TR-57, cycloheximide, or DMSO. (c) Cell viability plots for SUM159 cells transfected with siCTRL (black circles) or siClpP (white squares) using Hoechst stain for total cell count after 48-h treatment with ONC201 or TR-57. Graphs shown in (b) and (c) are representative of 2 biological replicates. Error bars represent standard error of the mean (SEM) from replicates.