Table 1.
Gene (GeneBank ID) | PCR primer sequences | Product length |
---|---|---|
β-actin (NM-173979.3) |
F:5′-CATCACCATCGGCAATGAGC-3′ R:5′-AGCACCGTGTTGGCGTAGAG-3’ |
156 bp |
IL-1α (NM-174092.1) |
F:5’CTAAAGGAGATGCCTGAGACACC-3′ R:5’CTGATTTGAAGTAGTCCATAGAGCC-3’ |
97 bp |
IL-1β (NM-174093.1) |
F:5’-TGATGACCCTAAACAGATGAAGAGC-3′ R:5’-CCACGATGACCGACACCACCT-3’ |
134 bp |
IL-6 (EU-276071.1) |
F:5’-TGAAAGCAGCAAGGAGACACT-3′ R:5′-TGATTGAACCCAGATTGGAAGC-3’ |
90 bp |
IL-8 (NM-173979.3) |
F:5’-TTCCTCAGTAAAGATGCCAATG-3′ R:5′-TGACAACCCTACACCAGACCCA-3’ |
86 bp |
TNF-α (AF-348421.1) |
F:5’-GGGCTTTACCTCATCTACTCACAG-3′ R:5′-GATGGCAGACAGGATGTTGACC-3’ |
132 bp |