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. 2019 May 21;15:157. doi: 10.1186/s12917-019-1912-y

Table 1.

Real-time PCR primers and product length

Gene (GeneBank ID) PCR primer sequences Product length

β-actin

(NM-173979.3)

F:5′-CATCACCATCGGCAATGAGC-3′

R:5′-AGCACCGTGTTGGCGTAGAG-3’

156 bp

IL-1α

(NM-174092.1)

F:5’CTAAAGGAGATGCCTGAGACACC-3′

R:5’CTGATTTGAAGTAGTCCATAGAGCC-3’

97 bp

IL-1β

(NM-174093.1)

F:5’-TGATGACCCTAAACAGATGAAGAGC-3′

R:5’-CCACGATGACCGACACCACCT-3’

134 bp

IL-6

(EU-276071.1)

F:5’-TGAAAGCAGCAAGGAGACACT-3′

R:5′-TGATTGAACCCAGATTGGAAGC-3’

90 bp

IL-8

(NM-173979.3)

F:5’-TTCCTCAGTAAAGATGCCAATG-3′

R:5′-TGACAACCCTACACCAGACCCA-3’

86 bp

TNF-α

(AF-348421.1)

F:5’-GGGCTTTACCTCATCTACTCACAG-3′

R:5′-GATGGCAGACAGGATGTTGACC-3’

132 bp