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. 2019 May 20;17:165. doi: 10.1186/s12967-019-1917-0

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© The Author(s) 2019

Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.

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Fig. 2 — Tim-3 expression is associated with apoptosis of tumor-infiltrating NK cells. a Correlation analysis between Tim-3⁺ NK cells and total NK cells in PBMC and TIL from esophageal cancer patients. b TIL were stained with antibodies to CD3, CD56, Tim-3 and Annexin V. The flow data are representative of 23 patients with esophageal cancer (left panel) and the results are shown as bar graphs (right panel). c NK cells in PBMC and TIL were examined for Annexin V binding, respectively. The flow data are representative of 14 patients with esophageal cancer (left panel) and the results are shown as bar graphs (right panel). ***p < 0.001; ns, not significant