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. Author manuscript; available in PMC: 2020 May 1.
Published in final edited form as: Neurobiol Aging. 2019 Jan 30;77:87–93. doi: 10.1016/j.neurobiolaging.2019.01.023

Figure 3. Depletion of Par3 increases colocalization of internalized APP with BACE1.

Figure 3.

(a) APPwt N2a cells were transfected with indicated constructs together with hBACE1-FLAG. 72h after transfection, internalized APP (60 min) was immunostained (red) together with FLAG (green) to visualize colocalization of internalized APP and BACE1. (b) N2a cells were transfected with indicated constructs together with hBACE1-FLAG. 72h after transfection, cells were immunostained for FLAG (red) and Presenilin-1 (PS1, green). Scale bar: 10μm. (c) Quantification of colocalization of internalized APP with BACE1-FLAG. N (cells) = 19 (myc), 17 (Par3b), 19 (Luciferase shRNA), 20 (Par3 shRNA) from 5 independent experiments. (d) Quantification of the colocalization between BACE1-FLAG and PS1. N (cells) = 12 (myc), 12 (Par3b), 12(Luciferase shRNA), 11 (Par3 shRNA) from 3 independent experiments. Data were expressed as Mean ± SEM with Student’s t-test: ** p < 0.01.