Fig. 1.

Dose dependent cisplatin (CP)-induced kidney injury and mortality in WT mice. 129 sv WT mice were intraperitoneally injected once with 0, 10, 20 mg/Kg CP and followed for up to 20 weeks after the injection. a Survival rate. b Plasma Cr. c Plasma Pi. d ACR. e Kidney histology assessed by HE, TC, and PSR staining (scale bar = 500 μm); and semi-quantitative assessment (bottom panel including chronic pathologic score based on HE stain and renal fibrosis score based on PSR stain). f Representative immunoblots for αKlotho protein in total kidney lysates (upper panel). Summary of all immunoblots (bottom panel). g αKlotho and NGAL mRNA expression in kidney lysates at one week after CP injection. Transcripts in the kidneys were analyzed with qPCR and results are expressed as 2^−ΔΔCt (Ct = cycle number) by normalization to cyclophilin and compared to the 0 mg/Kg group. Data are expressed as means ± SD from each group and statistical significance was evaluated by one-way ANOVA followed by Student–Newman–Keuls post hoc test, and significance was accepted when *P < 0.05; **P < 0.01 vs. 0mg/Kg, ^#P < 0.05 vs. 10mg/Kg for a–d, g; or when *P < 0.05; **P < 0.01 between two groups for f. h αKlotho and α-SMA mRNA expression in kidney lysates at 20 weeks after the injection. Data are expressed as means ± SD from each group and statistical significance was evaluated by unpaired Student-t-test and significance was accepted when *P < 0.05; **P < 0.01 between two groups for e and h. BUN blood urea nitrogen; Cr creatinine; CP cisplatin; HE Hematoxylin and Eosin stain; IP intraperitoneal injection; Pi plasma phosphate; TC Trichrome stain; PSR Picrosirius red stain