Figure 2. TEM and CD spectra of isolated ECM from S. aureus biofilm grown in PNG and exposed to GQDs.
(a) Amyloid fibrils in composition with ECM attached to bacteria; (b) Extracted composite ECM treated with (b) 0 μg/mL and (c) 50 μg/mL GQDs for 4 days, (d) CD spectra of isolated ECM treated with 0 μg/mL, 10 μg/mL, and 50 μg/mL GQD for 4 days. Scale bars: 500 nm (a), 100 nm (b, c). Only low concentrations of GQDs were evaluated in this test due to the low concentration of ECM obtained from the isolation procedure compared to that from intact biofilms.