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. Author manuscript; available in PMC: 2020 Jan 16.
Published in final edited form as: ACS Chem Neurosci. 2018 Sep 13;10(1):358–370. doi: 10.1021/acschemneuro.8b00287

Figure 4.

Figure 4.

(a) Pertussis toxin (PTX) does not affect the activity of VU0529331 on G2Y4 cells while (b) activation of GIRK2 through the Gβγ-subunit after GPCR activation is fully inhibited. The G2Y4 cells express neuropeptide Y receptor type 4 (NPY4R) in addition to any GPCRs native to HEK293 cells. NPY4R was activated using a maximally-effective concentration, 200 nM, of human pancreatic polypeptide (hPP). All values in (a) were normalized to the predicted maximum activity of VU0529331 under VHL conditions. VU0529331 activity in (b) is normalized data from the 30 μM value in (a). 95% confidence intervals for the EC50 measurements were calculated using a 2-tailed Mann-Whitney test. **** indicates p<0.0001. Error bars represent the standard error of the mean (SEM).