Table 2.
Emission/excitation wavelengths and filter sets for each fluorophore used during flow cytometric analysis.
| Fluorophores | Laser (excitation wavelength) | Emission maximum (nm) | Filter sets |
|---|---|---|---|
| APC/Cy7 | Red (633 nm) | 785 | 780/60 (755LP) |
| Alexa Fluor® 700 | Red (633 nm) | 719 | 730/45 (685LP) |
| Alexa Fluor® 647 | Red (633 nm) | 668 | 670/30 |
| PerCP/Cyanine 5.5 | Blue (488 nm) | 695 | 695/40 (685LP) |
| FITC | Blue (488 nm) | 519 | 530/30 (505LP) |
| PE/Cy7 | Blue (488 nm) | 785 | 780/60 (735LP) |
| PE/Dazzle 594 | Blue (488 nm) | 610 | 610/20 (600LP) |
| Brilliant Violet 510 | Violet (405 nm) | 510 | 585/42 (550LP) |
| Brilliant Violet 605 | Violet (405 nm) | 605 | 675/25 (595LP) |
| Brilliant Violet 421 | Violet (405 nm) | 421 | 450/50 |
| Zombie UV | Ultraviolet (355 nm) | 459 | 450/50 |