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. Author manuscript; available in PMC: 2020 Mar 22.
Published in final edited form as: J Nat Prod. 2019 Feb 22;82(3):500–509. doi: 10.1021/acs.jnatprod.8b00873

Figure 4. Tissue specific expression and activity of deconjugating enzymes (GUSB, HPSE, KL) in C57BL/6J mice.

Figure 4.

A) Curcumin-glucuronide specific deconjugation activity of GUSB, HPSE, or KL was determined by quantitation of curcumin-glucuronide substrate and curcumin product after incubating recombinant human enzymes with curcumin-glucuronide (n=3–6/group). **** p < 0.0001 vs control (or as specified). B) Immunoblot of GUSB, HPSE, and KL expression in indicated tissues from female, 4-week, C57BL/6J mice (representative of n=4 blots). C) Tissue-specific GUSB enzyme activity using 4-MUG in 4-week female C57BL/6J mice (n=4–5/group). ****p < 0.0001 vs marrow.