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. Author manuscript; available in PMC: 2020 Mar 22.
Published in final edited form as: J Nat Prod. 2019 Feb 22;82(3):500–509. doi: 10.1021/acs.jnatprod.8b00873

Figure 5. Effect of a naturally occurring loss of function GUSB mutation in C3H/HeJ mice (vs C57BL/6J mice) on marrow GUSB expression and activity and the capacity of marrow to hydrolyze curcumin-glucuronide distributing to bones following in vivo curcumin treatment.

Figure 5.

A) GUSB enzyme kinetics using phenolphthalein-glucuronide in marrow from female 4-week C57BL/6J vs. C3H/HeJ mice (n = 3/group). B) Immunoblot of GUSB protein in marrow from female 4-week C57BL/6J vs. C3H/HeJ mice (representative of n=4 biological replicate blots). C) Aglycone curcumin levels 30 minutes after oral gavage with 500 mg/kg curcumin in male 15-week C57BL/6J vs C3H/HeJ mice, expressed as % of total curcumin in serum vs. marrow suspensions. There were no significant differences in GUSB activity based on sex or age (data not shown). ****p < 0.0001 vs C57BL/6J.