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. Author manuscript; available in PMC: 2020 Mar 22.
Published in final edited form as: J Nat Prod. 2019 Feb 22;82(3):500–509. doi: 10.1021/acs.jnatprod.8b00873

Figure 6. Comparison of GUSB expression and activity in marrow from wild type vs. GUSB mutant mice and the capacity of marrow to hydrolyze curcumin glucuronide distributing to bones following in vivo curcumin treatment.

Figure 6.

A) GUSB enzyme activity in marrow (normalized to wild-type BL/6J) in 15-week male mice (n=3–5/group). B) Immunoblot of GUSB, HPSE, and KL expression in marrow from 15-week female wild-type C57BL/6J and partial loss-of-function C3H/HeJ mice, and 15-week male hetero- (+/mps) and homozygous (mps/mps) GUSB deficient mice (representative of n=3 biological replicate blots). C) Aglycone curcumin levels 20 minutes after IP curcumin administration (100 mg/kg) to 15-week female BL/6J, female HeJ mice and male mps/mps mice (n=3–5/group), expressed as % of total curcumin in serum vs. marrow suspensions. There were no significant differences in GUSB activity or aglycone curcumin content based on sex (data not shown). ****p < 0.0001 vs C57BL/6J. nd = not detectable