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. Author manuscript; available in PMC: 2020 Mar 22.
Published in final edited form as: J Nat Prod. 2019 Feb 22;82(3):500–509. doi: 10.1021/acs.jnatprod.8b00873

Figure 7. The role of extracellular bone marrow cell-derived GUSB in deconjugation of curcumin-glucuronide.

Figure 7.

A) GUSB activity in total bone marrow (BM), cell-associated (intracellular) BM fraction, and cell-free (extracellular) BM fraction from female 4-week C57BL/6J (n=3/group) B) Aglycone curcumin levels, expressed as % of total curcumin, after incubating total BM or extracellular BM fraction for 2 hours in sodium acetate buffer (50 mM, pH 5) after ex vivo addition of curcumin-glucuronide (0.5 μM) using female 4-week C57BL/6J (n=3/group). C) Aglycone curcumin levels produced after incubating 10 μM curcumin-glucuronide in fresh media + 10% FBS + 1% pen/strep or marrow-conditioned media (CM), from female 4-week C57BL/6NJ, with or without SL (10 mM) for 2 hours at 37°C (n=4–8/group). Inset is a representative chromatogram. D) Comparison of GUSB enzyme activity in bone marrow (vs. serum) and curcumin glucuronide substrate concentrations in bone marrow (collected ex vivo in acetate buffer containing SL [10mM]) vs serum collected 30 minutes after gavage of female 4-week C57BL/6J mice with 500 mg/kg curcumin. Data expressed as mean ± SEM.