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. 2019 Mar;22(3):302–309. doi: 10.22038/ijbms.2019.31972.7683

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This is an Open Access article distributed under the terms of the Creative Commons Attribution License, (http://creativecommons.org/licenses/by/3.0/) which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.

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(A) SDS-PAGE analysis of the recombinant polytope protein. Lane 1, 2, 3, 4, 5 and 6: bacterial lysate expressing the induced pET32a, pET32a/polytope before the induction, pET32a/polytope protein in 1, 2 and 3 hr after induction and protein marker, respectively. (B) Analysis of the recombinant polytope- interleukin-2 (IL-2) protein. Lane 1, 2, 3, 4, 5 and 6: the bacterial lysate containing pET32a/polytope-IL-2 before the induction, pET32a/polytope-IL-2 protein in 1, 2 and 3 hr after induction, protein marker and induced pET32a, respectively. (C) Analysis of the recombinant IL-2 protein. Lane 1, 2, 3, 4, 5 and 6: the bacterial lysate containing pET32a/IL-2 before the induction, pET32a/IL-2 protein in 1, 2, 3 and 4 hr after induction and protein marker