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(A) SDS-PAGE analysis of the purified recombinant proteins including interleukin-2 (IL-2), polytope and polytope-IL-2. (B) Dot-blot analysis using anti-His tag antibody. Lane 1: extract of the transformed bacteria uninduced by isopropyl β-D-1-thiogalactopyranoside (IPTG), Lane 2, 3 and 4: the extract of transformed bacteria (Escherichia coli/pET32a/polytope-IL-2, E. coli/pET32a/Polytope and E. coli/pET32a/IL-2) induced by IPTG
