Figure 5.

Effects of inhibitors on intestinal colonization. Mice were given the negative control strain (△ToxT), the wild-type strain containing a solvent control (DMSO), or the wild-type strain containing an inhibitor (0.1 mM virstatin, 0.1 mM compound 4, or 1 mM compound 8). A Box-Cox transformation of the colonization index raw data (output/input CFUs) was performed to normalize the data distribution (Figure S2). Pairwise means comparison using Tukey-Kramer HSD indicates statistically significant differences between 2 pairs: DMSO/△ToxT (p=0.0011) and DMSO/8 (p=0.0029). Note: A decrease in CI for compound 8 was also statistically significant when the untransformed (raw) data was analyzed using the nonparametric Kruskal-Wallis test (Figure S2).