Fig. 2.

Effects of filipin III on EGFR, Src, Akt, and ERK1/2 phosphorylation in EGF-stimulated cells. A, C9 cells were pretreated for 30 min with filipin III (5–50 μM) before 20 ng/ml EGF stimulation for 5 min. After treatment, the cell lysates were separated by SDS-PAGE and analyzed in immunoblots probed with antibodies against phospho-EGFR, phospho-Src, phospho-Akt, phospho-ERK1/2, and total RSK2. B, pooled data plot signal intensity of each data point as determined by densitometry. * P < 0.01 compared with EGF alone, taken as 100.