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. Author manuscript; available in PMC: 2020 Mar 15.
Published in final edited form as: Mol Cell Endocrinol. 2018 Dec 20;484:78–92. doi: 10.1016/j.mce.2018.12.011

Fig-2: Effects of ERK1/2 and AKT pathways on regulation of transcriptional factors in human endometriotic cells:

Fig-2:

Panel-1A: Representative Immunoblot. Panel 1B: Histogram. The human endometriotic epithelial cells 12Z and stromal cells 22B were treated with MEK1/2 inhibitor (U0126, 20μm) to suppress ERK1/2 pathway or PI3K inhibitor (LY294002, 50μm) to suppress AKT pathway for 24h. Expression of important downstream transcriptional factor proteins were analyzed by western blot. β-actin protein was measured as an internal control. The densitometry of autoradiograms was performed using an Alpha Imager. Data expressed in integrated density value (IDV). *- control vs. treatment, p<0.05, n=3. See Materials and Method section for additional experimental details.