Table 2.
Gene | Primer sequence (5′ to 3′) | Accession | |
---|---|---|---|
CD49f | Forward | GATGCTGCCAACGCTGTATTC | NM_001277970 |
Reverse | GCCGTTCTGGCAACAGATG | ||
c‐Myc | Forward | TGCGGTCGCTACGTCCTT | NM_010849 |
Reverse | TCCAAGTAACTCGGTCATCATCTC | ||
Oct4 | Forward | CCGTGTGAGGTGGAGTCTGGAG | NM_013633 |
Reverse | GCGATGTGAGTGATCTGCTGTAGG | ||
NGFR | Forward | CAGGGAAACATCTGGAAACGA | NM_033217 |
Reverse | TGGACCAGGTTTTGAACAGACA | ||
Smo | Forward | AAGGCCACCCTGCTCATCTG | NM_176996 |
Reverse | AGGCCTTGGCGATCATCTTG | ||
Nanog | Forward | AGGACAGGTTTCAGAAGCAGAAGT | NM_028016 |
Reverse | TCAGACCATTGCTAGTCTTCAACC | ||
Klf4 | Forward | ACTATGCAGGCTGTGGCAAAA | NM_01063 |
Reverse | CCGTCCCAGTCACAGTGGTA | ||
Bmi1 | Forward | CGCTCTTTCCGGGATCTTTT | NM_007552 |
Reverse | CCCTCCACACAGGACACACA | ||
Twist | Forward | AGAAGAGCAGAGACCAAATTCACA | NM_011658 |
Reverse | GCTGCCCCTCTGGGAATC | ||
Nestin | Forward | GGCATCCCTGAATTACCCAA | NM_016701 |
Reverse | AGCTCATGGGCATCTGTCAA | ||
Lsd1 | Forward | GTTCATCAGGAATCGCACATTG | NM_001347221 |
Reverse | GCTGTTGTAAGGCGCTTCCA | ||
Sox9 | Forward | GCAGACCAGTACCCGCATCT | NM_011448 |
Reverse | CCTCCACGAAGGGTCTCTTCT | ||
Patched1 | Forward | CGAGACAAGCCCATCGACATTA | NM_001328514 |
Reverse | AGGGTCGTTGCTGACCCAAG | ||
Cdh2 | Forward | AGCGCAGTCTTACCGAAGG | NM_007664 |
Reverse | TCGCTGCTTTCATACTGAACTTT | ||
Fn1 | Forward | GATGTCCGAACAGCTATTTACCA | NM_001276408 |
Reverse | CCTTGCGACTTCAGCCACT | ||
Vim | Forward | CGTCCACACGCACCTACAG | NM_011701 |
Reverse | GGGGGATGAGGAATAGAGGCT | ||
Tgfb1 | Forward | CTCCCGTGGCTTCTAGTGC | NM_011577 |
Reverse | GCCTTAGTTTGGACAGGATCTG | ||
a‐Sma | Forward | GTC CCA GAC ATC AGG GAG TAA | NM_007392 |
Reverse | TCG GAT ACT TCA GCG TCA GGA | ||
Gapdh | Forward | AGGTCGGTGTGAACGGATTTG | NM_001289726 |
Reverse | TGTAGACCATGTAGTTGAGGTCA |