Figure 4.

Osteogenic differentiation of BMSCs on titanium surfaces in vitro. (A) ALP activity of BMSCs cultured on Ti, SLA, and direct metal laser sintering (DMLS) titanium surfaces for 7 and 14 days. (B) The relative mRNA levels of Runx2 in BMSCs cultured in the designated conditions on different materials for 7 and 14 days. (C) The relative mRNA levels of OC in BMSCs cultured according to the different groups for 7 and 14 days. (D) Immunofluorescence staining assays for Runx2 in BMSCs (red, Runx2; blue, DAPI) cultured on different samples for 7 and 14 days. (E) The total protein expression of Runx2 in BMSCs at 14 days after osteoinduction was examined by western blot. Error bars represent SD (n = 3). *P < .05. **P < .01