Figure 6.

Epigenetic regulation of BMSCs on the direct metal laser sintering (DMLS) titanium surface. (A) Immunofluorescence staining for H3K4me3 in BMSCs (red, H3K4me3; blue, DAPI) cultured on Ti, SLA and DMLS titanium surfaces for 7 and 14 days. (B) Protein expression of H3K4me3 at day 14 after osteoinduction. (C) ChIP assay of the H3K27me3 level at the promoter area of the osteogenic gene Runx2 at 0, 1, 2, 3 days. (D) ChIP assay of the H3K4me3 level at the promoter area of the osteogenic gene Runx2 at 7 days and 14 days. Error bars represent SD (n = 3). **P < .01