Figure 3.

Hox transcript antisense intergenic RNA (HOTAIR) mediated RCC migration, invasion in vitro and tumorigenesis in vivo. A, Wound healing assays were performed to investigate changes in cell migration. B, Transwell assays were carried out in ACHN and 786‐O cells. C, Xenografted tumours injected with 786‐O cells were measured to evaluate the cell proliferation in vitro. D, Above: HE staining of xenografted tumours. Blow: IHC staining with ki67 antibody. E, Xenografted tumours injected with ACHN cells were measured to evaluate the cell proliferation in vitro. F, Above: HE staining of xenografted tumours. Blow: IHC staining with ki67 antibody. Each experiment was independently repeated at least three times. Data were presented as mean ± SD (A‐B n = 3, C‐F n = 6). *P < 0.05