Figure 5.

Alpha‐2,8‐sialyltransferase 4 (ST8SIA4) promotes the RCC cell proliferation and metastasis. A, The expression of ST8SIA4 was detected in RCC tissues, corresponding nontumour tissues, and RCC cell lines. B, Immunohistochemistry (IHC) staining was performed to analysis the ST8SIA4 expression in RCC tissues and corresponding nontumour tissues. (C and D) The relative levels of ST8SIA4 mRNA and protein were detected by qRT‐PCR and Western blot in 786‐O cells transfected with ST8SIA4 or siST8SIA4. (E and F) Upregulated ST8SIA4 significantly increased colony formation and immunofluorescence intensity with EdU. (G, H and I) The nude mice were injected with 786‐O cells transfected with ST8SIA4 or shST8SIA4, the xenografted tumours were measured and IHC staining was performed. (J and K) The wound healing and cell invasion assays stimulated by ectopic expression of ST8SIA4 could be repressed by knockdown of siST8SSIA4. Each experiment was independently repeated at least three times. Data were presented as mean ± SD (A‐F, J, K n = 3 and G‐I n = 6). *P < 0.05