Table 2.
Primers used for quantitative RT‐PCR analysis
| Target gene | Gene abbreviation | Primer sequence | NBCI reference | |
|---|---|---|---|---|
| Forward | Reverse | |||
| Glyceraldehyde 3‐phosphate dehydrogenase | GAPDH | AGCCTCAAGATCATCAGCAA | GTCATGAGTCCTTCCAGGAT | NM_002046.5 |
| Collagen, type I, α1 | COL1A1 | GTCACAGATCACGTCATCGC | CGAAGACCCACCAATCAC | NM_000088.3 |
| Collagen, type III, α1 | COL3A1 | TTGGCATGGTTCTGGCTTCC | GCTGGCTACTTCTCGTG | NM_000090.3 |
| Osteogenic‐related markers | ||||
| Secreted phosphoprotein 1 (Osteopontin) | SPP1 | CAGACCTGACATCCAGTACCC | GGTCATCCAGCTGACTCGTT | NM_001040058.1 |
| Run‐ related transcription factor 2 | RUNX2 | TGTCTGTGCCTTCTGGGTTC | CCGGCCTGCCTATGCTGTTA | NM_001024630.3 |
| Alkaline Phosphatase | ALPL | GAAGGAAAAGCCAAGCAGGC | GGGGGCCAGACCAAAGATAG | NM_000478.5 |
| Tenogenic‐related markers | ||||
| Scleraxis | SCX | CGAGAACACCCAGCCCAAAC | CTCCGAATCGCAGTCTTTCTGTC | NM_001717912 |
| Mohawk | MKX | TGTTAAGGCCATAGCTGCGT | TCGCACAGACACCTGGAAAA | NM_173576.5 |
| Decorin | DCN | CAGCATTCCTCAAGGTCTTCCT | GAGAGCCATTGTCAACAGCA | NM_001920.3 |
| Tenascin‐c | TNC | ACTGCCAAGTTCACAACAGACC | CCCACAATGACTTCCTTGACTG | NM_002160.3 |
| Chondrogenic‐related markers | ||||
| Cartilage oligomeric matrix protein | COMP | AGGATGGAGACGGACATCAG | TCTGCATCAAAGTCGTCCTG | NM_000095.2 |
| Aggrecan | ACAN | TGGTCTTGCAGCAGTTGATTC | TAGAGTCCTCAAGCCTCCTGT | NM_013227.3 |