Figure 1.

1,25(OH)₂D₃ inhibits the Th17 cells in vitro. A, The cell proportion of purified naive CD4⁺ T cells before or after isolation was tested by flow cytometry. B, Cell viability of Th17 cells in the presence of 1, 10, or 100 nmol/L 1,25(OH)₂D₃ or vehicle was tested by CCK8 assay. C, Apoptosis of Th17 cells under the presence of 10 nmol/L 1,25(OH)₂D₃ or vehicle for 3 d was analysed by flow cytometry. D, The purified CD4⁺ T cells from WT mice and VDR−/− mice were treated with 10 nmol/L 1,25(OH)₂D₃ and the proportion of CD4⁺/IL‐17⁺ Th17 cells was analysed by flow cytometry. NS P > .05, *P < .05, ***P < .001