Figure 4.

1,25(OH)₂D₃ inhibited Th17 cells in vitamin D deficiency mouse model. ELISA assay was performed to test the level of VD (A), RANKL (B) and IL‐17 (C) in the blood. Q‐PCR assessed the mRNA level of IL‐17, RORγt and RANKL in spleen (D‐F). (G) Western blot analysed the protein level of IKB, cytoplasm P65, nuclear P65, IL‐17, RORγt and RANKL in Th17 cells. (H) Densitometry plot of results from Figure 4G. The relative expression levels were normalized to GAPDH. Data represent the mean ± standard error (n = 3). *P < .05, **P < .01, ***P < .001 vs VDR−/− group. ^## P < .01, vs WT group