Figure 1.

Odontoblastic differentiation of human dental pulp cells (hDPCs). A, The expression of the odontoblastic markers DSPP and DMP1 was assessed using Western blotting; the band intensities were analysed using ImageJ software; GAPDH was used as an internal control. B, Alizarin red S staining of hDPCs on day 0, 7 and 14 of culture in odontoblastic induction medium. The “black” scale bars represent 100 μm (original magnification ×100). C, The FAM20C protein was detected using Western blot analysis, and the band intensities were analysed using ImageJ software; GAPDH was used as an internal control. All of the results represent the mean ± standard deviation of 3 independent experiments (n = 3). *Significant difference compared with the control (P < .05)