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. 2017 Dec 25;51(2):e12426. doi: 10.1111/cpr.12426

Figure 2.

Figure 2

Effect of FAM20C knockdown on the odontoblastic potential of human dental pulp cells. A, The expression level of FAM20C in FAM20C‐shCtrl and FAM20C‐shRNA groups was assessed using Western blotting. The band intensities were analysed using ImageJ software; GAPDH was used as an internal control. B, ALP activity in the odontoblastic differentiation medium at day 7 was determined in FAM20C‐shCtrl and FAM20C‐shRNA groups. C, The protein levels of odontoblastic markers in FAM20C‐shCtrl and FAM20C‐shRNA groups after 7 and 14 days of odontoblastic induction were assessed using Western blotting. The band intensities were analysed using ImageJ software; GAPDH was used as an internal control. D, Mineralization was analysed using alizarin red S staining. The formation of mineralized nodules was analysed at day 14 in FAM20C‐shCtrl and FAM20C‐shRNA groups undergoing odontoblastic induction. The “black” scale bars represent 100 μm (original magnification ×100). All of the results represent the mean ± standard deviation of 3 independent experiments (n = 3). *Significant difference compared with the control (P < .05)