Figure 5.

TET1 directly binds to FAM20C and engages its hydroxymethylation. A, Promoter region of the FAM20C gene. Arrows and numbers show the location of primers used in these analyses. The long CpG island is shown in orange. B, Dynamic hydroxymethylation levels of IP‐DNA were evaluated with qPCR using the corresponding primer pairs indicated above. Bars represent shCtrl (no stripes) and shTET1 (stripes) groups. C, Signal of TET1 IP‐DNA relative to the total amount of input DNA in human dental pulp cells extracted before (no stripes) and after (stripes) odontoblastic induction, as analysed with qPCR using the specific primer pairs indicated in (A). D, The significant results of (C) were verified via agarose gel electrophoresis. All of the results represent the mean ± standard deviation of 3 independent experiments (n = 3). *Statistically significant difference compared with the control; P < .05