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. 2018 Aug 28;51(6):e12515. doi: 10.1111/cpr.12515

Figure 4.

Figure 4

LncRNA SNHG4 acted as a sponge of miR‐224‐3p in osteosarcoma cells. (A) Pearson correlation analysis of the correlation of SNHG4 expression with miR‐224‐3p in patients with osteosarcoma. (B) Schematic representation of the binding sites of miR‐224‐3p with WT or Mut SNHG4. (C) The luciferase activity of WT or Mut SNHG4 was assessed after transfection with miR‐224‐3p mimic in Ssos‐2 and U‐2 OS cell lines. (D) qRT‐PCR analysis of the transfection efficiency of miR‐224‐3p mimic in Ssos‐2 and U‐2 OS cell lines. (E) qRT‐PCR analysis of the effects of SNHG4 overexpression on the expression of miR‐224‐3p in Ssos‐2 and U‐2 OS cell lines. (F) Ago2 RIP assay analysis of the enrichment of SNHG4 and miR‐224‐3p pulled‐down from the Ago2 protein in Ssos‐2 and U‐2 OS cell lines, and the expression levels of SNHG4 and miR‐224‐3p were examined by qRT‐PCR analysis. Data are the means ± SEM of three experiments. *P < 0.05, **P < 0.01