Figure 2.

Rigid substrates promote odontogenic differentiation. A, Transcriptional levels of DSPP andDMP‐1 in DPSCs at 7 days and 14 days post‐seeding by q‐PCR. Data have been firstly normalized to GAPDH, and then normalized to that of group 10:1. The results shown are representative of 3 different samples (n = 3). Data are presented as mean ± SD, *P<.05,**P<.01. B, Protein expression of DSPP and DMP‐1 was measured by Western blotting in DPSCs after culturing for 14 days and 21 days. C, Immunofluorescence of DSPP and DMP‐1 expressed in DPSCs cultured on different elastic substrates (from 10:1 to 40:1). Scale bars are 50 μm