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. 2018 Aug 17;51(5):e12470. doi: 10.1111/cpr.12470

Figure 5.

Figure 5

BMAL1 upregulates OPG expression by directly binding to the Opg promoter. (A‐B) Representative images of TRAP staining of RAW 264.7 cells co‐cultured with BMAL1‐knockdown mBMSCs or MC3T3‐E1 cells in the absence or presence of recombinant OPG. (magnification, 400x). (C) OPG concentration in the supernatants of co‐cultured RAW 264.7 cells quantified by enzyme‐linked immunosorbent assay. (D) The transcription factor BMAL1 bound to the Opg promoter in MC3T3‐E1 cells. Chromatin immunoprecipitation assays were performed using anti‐IgG as a negative control. (E) Luciferase reporter assays were performed to measure the activities of the wild‐type or mutated BMAL1‐binding site at the Opg promoter in BMAL1‐overexpressing or BMAL1‐knockdown MC3T3‐E1 cells. N = 3 independent experiments, data represent the mean ± SD, *P < .05, **P < .01, ***P < .001. Analysis of variance with Tukey's post‐hoc test was used