Figure 4.

The anti‐tumour effect of miR‐195 was impaired by LINC00473. (A) The expression of miR‐195 and its correlation to LINC00473 were analysed in Wilms tumour and relatively normal tissues were by Q‐PCR. (B) After the transfection of miR‐195 mimics and/or pcDNA 3.1‐LINC00473 in SK‐NEP‐1 cells, the cell vitality was estimated by CCK‐8. (C and D) the proliferation and apoptosis were determined by EdU assay and Hoechst assay. (E) The cell cycle was also analysed by flow cytometry. (F) The ability of migration and invasion was assessed by transwell assay. (G) The expression of Bcl‐2, caspase‐3, CDK2 and cyclin D1. *P < .05, **P < .01, ***P < .001, data represent the mean ± SD