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. 2018 Jul 23;51(5):e12477. doi: 10.1111/cpr.12477

Figure 1.

Figure 1

Expression profile of SPRY1 in human normal skin, NHEK and forced overexpression in NHEK. A, Immunohistochemistry staining of SPRY1, involucrin and isotype IgG in normal skin of healthy donors’ skin biopsy. Scale bar = 100 μm, SC: stratum corneum, SG: stratum granular; SS: stratum spinosum; SB: stratum basale; B, Immunofluorescence staining of SPRY1 (green) and Involucrin (red) in normal skin of healthy donors’ skin biopsy. Scale bar = 20 μm. Dotted line indicated the SG and SS. C, Immunofluorescence staining of SPRY1 and isotype IgG in primary NHEK which was obtained from healthy donor. Scale bar = 50 μm. Green: SPRY1 protein; Blue: DAPI; Red dot circle notes SPRY1 specific high fluorescence of NHEK, yellow dot circle notes SPRY1‐specific low fluorescence of NHEK. AC, Primary antibody of mouse IgG was used as negative control compared to SPRY1 antibody. D, Represented photos of cultured NHEK in different culturing status: sub‐confluence, confluence and post‐confluence. Scale bar = 20 μm. E, Western blot of SPRY1, involucrin and loricrin expression in NHEK with different cell confluence status. F, qRT‐PCR analyses SPRY1 and differentiation‐associated genes expression in NHEK. (**P < .01)