Figure 1.

Coroglaucigenin inhibits cell proliferation independent of apoptosis in colorectal cancer cells. (A) The structure of coroglaucigenin (CGN). (B) Cell viability was measured using MTT assays in HT‐29, SW480 and NCM460 cells treated with the indicated concentrations of CGN for 24 hours. (C) Cells were treated with the indicated concentrations of CGN for 24 hours, and the degree to which CGN inhibited cell proliferation was measured using BrdU labelling. (D and E) Cells were treated with the indicated concentrations of GCN for 24 hours, and the level of apoptosis was determined using an Annexin‐V‐FITC/PI double staining assay. (F) The level of cleavage of PARP was determined using western blotting in HT‐29 and SW480 cells treated as in D. (G) ImageJ densitometric analysis of the cleaved PARP/β‐actin ratios from immunoblots. *P  < 0.05, **P < .01, ***P < .001