Figure 3.

Coroglaucigenin induces protective autophagy in colorectal cancer cells. (A) Immunoblot analysis of LC3 in HT‐29 and SW480 cells treated with the indicated concentrations of CGN for 24 hours. Protein ratios were calculated following ImageJ densitometric analysis. (B) Immunoblot analysis of LC3 in cells treated with the indicated concentrations of CGN for 24 hours in the absence or presence of 3‐methyladenine (3‐MA). (C and D) HT‐29 and SW480 cells were treated with DMSO or 4 μM of CGN for 24 hours, and the formation of endogenous LC3 puncta (arrows) was visualized under a fluorescent microscope. Scale bars, 10 μm. (E) Cell viability was measured by MTT assays in cells treated with DMSO or 4 μM of CGN for 24 hours in the presence and absence of chloroquine (CQ). (F) Cell proliferation was measured using BrdU labelling in cells treated with DMSO or 4 μM of CGN for 24 hours in the presence or absence of CQ. *P  < .05, **P  < .01