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Expression of TK1 was directly regulated by E2F‐1. A, Western blot and RT‐PCR were used to validate the interference efficiency of three siRNAs for E2F‐1. B, The expression of TK1 was downregulated by siRNA for E2F‐1. C, The promoter of TK1 could be combined with E2F‐1, and TK1 expression was decreased in E2F‐1 knockdown group. D, The proliferation PDAC cells were affected after E2F‐1 knockdown. ** represents P < .01, and *** represents P < .001
