Figure 4.
Ang II promoted the nuclear accumulation of YAP. A, Cultured rat VSMCs were incubated with 10−7 mol/L Ang II for the indicated times. The cells were subjected to immunofluorescence staining for YAP (red) and F‐actin (Alexa Fluor® 488 Phalloidin, green). All sections were counterstained with DAPI to visualize the nuclei (blue). Immunofluorescence images were acquired using a confocal microscope (Zeiss). Scale bars represent 10 μm. B, Rat VSMCs were treated with or without Ang II (10−7 mol/L) for 30 min. Nuclear and cytoplasmic fractions were extracted, and the protein levels of YAP in different fractions were measured by Western blot analysis. C, Quantification of YAP level in nuclear and cytoplasmic fractions in (B). The protein levels of YAP in nuclear or cytoplasmic fractions were normalized to H3 or GAPDH, respectively, and compared to that in the PBS control (set as 1). All results were presented as the mean ± SEM of three independent experiments performed in triplicate. *P < 0.05 compared with the control group, **P < 0.01 compared with the control group. GAPDH, glyceraldehyde phosphate dehydrogenase