Figure 4.

Cell adhesion, outgrowth and differentiation of Oct4‐GFP EBs cultured in space and ground environments. A, Formation of Oct4‐GFP EBs was used to analyse the characteristics of spontaneous differentiation during spaceflight. B, Representative bright field images at low magnification showing EB morphologies and outgrowth from day 1 to day 12 in culture. C, D, Phase contrast and GFP fluorescence images showing cell morphologies and Oct4 expression for the differentiation of Oct4‐GFP EBs over 15 days of culture in spaceflight and ground 1 × g conditions. All scale bars = 200 μm. E, Mean fluorescence intensity of spaceflight‐cultured and ground‐cultured OCT4‐GFP EBs. All results are shown as the mean ± SD of at 3 independent images. NS = no significant, (*) = P < .05, (***) = P < .001