Figure 1.

Characteristics of LNGFR ⁺ and LNGFR ⁻ EMSCs. (A) Flow cytometry sorting of LNGFR ⁺ and LNGFR ⁻ EMSCs. The cells in right section (P1) represent the LNGFR ⁻ EMSCs, while the cells in left section (P3) represent the LNGFR ⁺ EMSCs. (B) The morphologies of the LNGFR ⁺ and LNGFR ⁻ EMSCs were observed by optical microscopy (scale bar = 50 μm). (C, D) The flow cytometry detection of (C) mesenchymal stem cell surface markers (CD29, CD44, CD90 and CD146) and (D) hematopoietic markers (CD45 and CD34) on LNGFR ⁺ and LNGFR ⁻ EMSCs. (E, F) The (E) qPCR and (F) Western blot analysis of LNGFR mRNA and protein levels, respectively, in LNGFR ⁺ and LNGFR ⁻ EMSCs using GAPDH as a control. ***P < .001