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. 2017 Dec 10;51(2):e12412. doi: 10.1111/cpr.12412

Figure 3.

Figure 3

SOST negatively regulates the osteogenic differentiation of EMSCs. (A, B) The (A) qPCR and (B) Western blot analysis of SOST mRNA and protein levels, respectively, in LNGFR + and LNGFR − EMSCs using GAPDH as a control. (C‐E) The LNGFR − EMSCs were transfected with siSOST or siNC for 24 h and then treated with osteogenic induction medium an additional 7 days. On day 0 and day 7, the (C, D) mRNA and (E) protein levels of ALP and RunX2 were detected by qPCR and Western blot, respectively, using GAPDH as a control. (F‐H) The LNGFR + EMSCs were transfected with pMYC‐SOST or pMYC‐NC for 24 h and then treated with osteogenic induction medium for an additional 7 days. On day 0 and day 7, the (F, G) mRNA and (H) protein levels of ALP and RunX2 were detected by qPCR and Western blot, respectively, using GAPDH as a control. siSOST, siRNA for SOST; siNC, negative control siRNA; pMYC‐SOST, SOST expression plasmid; and pMYC‐NC, control plasmid; *P < .05, **P < .01, and ***P < .001