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. 2017 Oct 22;51(2):e12399. doi: 10.1111/cpr.12399

Figure 4.

Figure 4

CD4+ T cell‐primed GC‐MSCs have greater GC cell migration‐promoting role than GC‐MSCs in vitro. (A) Ability of GC‐MSCs‐CM and CD4+ T cell‐primed GC‐MSCs‐CM to induce SGC‐7901 cell migration was evaluated by Transwell migration assay (n = 3). Migrated cells on the lower layer were stained and photographed. The number of migrated cells was counted and presented as columns. Scale bar = 100 μm. **P < .01. (B) After co‐culture with control CM, GC‐MSCs‐CM and CD4+ T cell‐primed GC‐MSCs‐CM for 48 hours, SGC‐7901 cells were collected and stained with annexin V/PI (n = 4). Columns represented the ratio of annexin V and PI double‐positive cells. ns, no significant difference. (C) Colony forming assay of SGC‐7901 cells after pre‐treatment with control CM, GC‐MSCs‐CM and CD4+ T cell‐primed GC‐MSCs‐CM (n = 3). Columns represented the number of colonies. ns, no significant difference